Tired of playing second fiddle to Bugs Bunny, Daffy Duck demands his own movie from the Warner Bros., but they promptly fire him. Aspiring stuntman DJ Drake is asked to escort Daffy off the studio lot, but the ensuing chase leads to the Batmobile demolishing the studio water tower. DJ is fired, and returns home with Daffy hitching a ride, discovering his father and action star Damian Drake is actually a secret agent. Damian instructs his son to travel to Las Vegas, find his associate Dusty Tails, and gain the mystical Blue Monkey diamond before being captured by the Acme Corporation, led by the childish Mr. Chairman. DJ and Daffy head for Vegas. Meanwhile, Bugs’ routines fall apart without Daffy, so V.P. for Comedy, Kate Houghton, is sent to rehire Daffy or face being fired herself. Bugs informs her of the situation and they pursue DJ and Daffy in Damian’s spy car.
In Las Vegas, DJ and Daffy meet Dusty in a casino owned by Acme operative Yosemite Sam. Dusty gives them a strange playing card, but when Yosemite tries to kill them, they flee in the spy car with Bugs and Kate, but when Daffy cries “Mother!”, the car takes flight. Crashing in Death Valley, the group stumble across the secretive Area 52, run by Damian’s gadget specialist Mother. Mother plays a video recording, revealing Acme intends on using the Blue Monkey to transform mankind into monkeys to manufacture their products and then buy them as humans. Marvin the Martian, imprisoned in the facility, escapes and leads a group of fellow alien inmates to obtain the playing card but the heroes escape. Realising the card has the Mona Lisa’s face on it, the group conclude they must view the painting in the Louvre, Paris.